1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression
| Autor: | Jiang, Jiang, Zhang, Yang, Indra, Arup K, Ganguli-Indra, Gitali, Le, Mai N, Wang, Hongjun, Hollins, Ronald R, Reilly, Debra A, Carlson, Mark A, Gallo, Richard L, Gombart, Adrian F, Xie, Jingwei |
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| Rok vydání: | 2018 |
| Předmět: |
Keratinocytes
alpha Medical Biotechnology Nanofibers Bioengineering antimicrobial peptides LL-37 Monocytes Transgenic Cell Line Mice Anti-Infective Agents 25-dihydroxyvitamin D3 Animals Humans Nanotechnology sustained delivery Vitamin D Nanoscience & Nanotechnology innate immunity Wound Closure Techniques 1α Bandages 25-dihydroxyvitamin D-3 endogenous production electrospun nanofibers Antimicrobial Cationic Peptides Physical Chemistry (incl. Structural) |
| Zdroj: | Nanomedicine (London, England), vol 13, iss 12 |
| Popis: | AimThe aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction.Materials & methodsNanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo.Results1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants.Conclusion1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production. |
| Databáze: | OpenAIRE |
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