Sodium Butyrate Enhances Intestinal Riboflavin Uptake via Induction of Expression of Riboflavin Transporter-3 (RFVT3)
| Autor: | Subramanian, Veedamali S, Sabui, Subrata, Heskett, Christopher W, Said, Hamid M |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Colon Riboflavin Clinical Sciences RFVT3 Inbred C57BL Mice Genetic Genetics Animals Humans 2.1 Biological and endogenous factors Intestinal Mucosa Aetiology Gastroenterology & Hepatology Membrane Transport Proteins Sodium butyrate Biological Transport DNA Methylation Up-Regulation Intestine Organoids Colonoids Butyric Acid Caco-2 Cells Digestive Diseases Epigenesis |
| Zdroj: | Digestive diseases and sciences, vol 64, iss 1 |
| Popis: | BackgroundUptake of riboflavin (RF) by intestinal epithelial cells occurs via a specific carrier-mediated process that involves the apically localized RF transporter-3 (RFVT3). Previous studies have shown that sodium butyrate (NaB) affects intestinal uptake of other substrates and expression of their membrane transporters, but its effect on intestinal uptake of RF and expression of RFVT3 has not been examined.AimsTo investigate the effect of NaB on intestinal RF uptake process and expression of the RFVT3.MethodsTwo experimental models were used in this study: Human-derived intestinal epithelial Caco-2 cells and ex vivo mouse colonoids. 3H-RF uptake assay, Western blot, RT-qPCR, and chromatin immunoprecipitation assay were performed.ResultsTreating Caco-2 cells with NaB led to a significant increase in carrier-mediated RF uptake. This increase was associated with a significant induction in the level of expression of the hRFVT3 protein, mRNA, and heterogenous nuclear RNA (hnRNA). Similarly, treating mouse colonoids with NaB led to a marked increase in the level of expression of the mRFVT3 protein, mRNA, and hnRNA. NaB did not affect hRFVT3 mRNA stability, rather it caused significant epigenetic changes (histone modifications) in the SLC52A3 gene where an increase in H3Ac and a reduction in H3K27me3 levels were observed in the NaB-treated Caco-2 cells compared to untreated controls.ConclusionThese findings demonstrate that NaB up-regulates intestinal RF uptake and that the effect appears to be mediated, at least in part, at the level of transcription of the SLC52A3 gene and may involve epigenetic mechanism(s). |
| Databáze: | OpenAIRE |
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