Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
| Autor: | Gonçalves R. C. R., Belmonde-Reche, E., Pina, J., da Silva, M. C., Pinto, S. C. S., Gallo, J., Costa, Susana P. G., Raposo, M. Manuela M. |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Popis: | The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules27228065/s1, Synthesis and characterization of BODIPYs 1-3 and Figure S1: Comparison between the absorption and fluorescence emission spectra of anthracene and BODIPY 1 in tetrahydrofuran solutions at room temperature; Figure S2: Normalized absorption and fluorescence emission spectra for the investigated BODIPY derivatives in acetonitrile solution at room temperature. Fluorescence-based probes represent a powerful tool for noninvasive imaging of living systems in real time and with a high temporal and spatial resolution. Amongst several known fluorophores, 3-difluoroborodipyrromethene (BODIPY) derivatives have become a cornerstone for innovative fluorescent labelling applications, mainly due to their advantageous features including their facile synthesis, structural versatility and exceptional photophysical properties. In this context, we report a BODIPY-based fluorescent probe for imaging of lysosomes in living cells. The BODIPY derivative displayed a remarkable fluorescence enhancement at low pH values with a pKa* of 3.1. In vitro studies by confocal microscopy in HeLa cells demonstrated that the compound was able to permeate cell membrane and selectively label lysosome whilst remaining innocuous to the cell culture at the maximum concentration tested. Herein, the BODIPY derivative holds the promise of investigating lysosomal dynamics and function in living cells through fluorescence imaging. This research was funded by Fundação para a Ciência e Tecnologia (FCT) and FEDER (European Fund for Regional Development)-COMPETE-QRENEU through the Chemistry Research Centre of the University of Minho (ref. CQ/UM (UID/QUI/00686/2020) and the Coimbra Chemistry Centre (refs. UIDB/00313/2020 and UIDP/00313/2020), project PTDC/QUI-COL/28052/2017 and a PhD grant of R.C.R. Gonçalves (SFRH/BD/05278/2020). The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network and was purchased within the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT. |
| Databáze: | OpenAIRE |
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