Monoolein as helper lipid for non-viral transfection in mammals

Autor:	Oliveira, Ana Cristina Norberto Gonçalves, Silva, J. P. Neves, Coutinho, Paulo J. G., Gomes, A. A., Coutinho, O. P., Oliveira, M. Elisabete
Přispěvatelé:	Universidade do Minho
Jazyk:	angličtina
Rok vydání:	2010
Předmět:	Lipoplexes DODAB Monoolein Science & Technology lipids (amino acids peptides and proteins) Transfection
Popis:	Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells. The Portuguese Foundation for Science and Technology (FCT) for the financial support to the Center of Physics and Center of Molecular & Environmental Biology and funding through projects PTDC/QUI/69795/2006 and SFRH/BD/46968/2009 are acknowledged.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od_______307::7e8d5026d7f3f8aa316767862e953e88 https://hdl.handle.net/1822/14095 Zobrazit plný text záznamu