Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4
| Autor: | Braga, Adelaide, Gomes, Daniela Filipa Correia, Costa, João Manuel Rainha, Cardoso, Beatriz, Amorim, Cláudia, Silvério, Sara Isabel Cruz, Fernández-Lobato, María, Rodrigues, Joana Lúcia Lima Correia, Rodrigues, L. R. |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Popis: | The online version contains supplementary material available at https://doi.org/10.1007/s00253-022-12037-3 Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated -fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4±1.6 g L1 of FOS, with a productivity of 1.53±0.03 g L1 h1 and a yield of 0.31±0.03 gFOS gsucrose1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process. s Cláudia Amorim, João Rainha, Beatriz B. Cardoso, and Daniela Gomes acknowledge their grants (UMINHO/ BPD/4/2019, SFRH/BD/138325/2018, SFRH/BD/132324/2017, and SFRH/BD/04433/2020, respectively) from Portuguese Foundation for Science and Technology (FCT). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|