Evaluating the in vitro digestion of lipids rich in medium-chain fatty acids (MCFAs) using dynamic and static protocols
| Autor: | Pereira, Ericsem, Fernandes, Jean Michel, Gonçalves, Raquel F. S., Pinheiro, A. C., Duarte, Maria Salomé, Alves, M. M., Meirelles, Antonio J. A., Maximo, Guilherme J., Vicente, A. A. |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
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| Popis: | Investigating the digestion of lipids is paramount for developing new lipid-based products. This work evaluated the gastrointestinal (GI) digestion of medium-chain fatty acids (MCFAs) rich lipids. The dynamic GI in vitro system was used to simulate gastric, duodenal, jejunal, and ileal GI tract portions. Results from the dynamic protocol were compared against static in vitro assays and GC analyses were conducted to assess the FA profile of FFA released during digestion. Caprylic and capric acids released during the gastric digestion of MCT oil varied from 6163% and 3638% of total esterified FA, respectively. Lauric acid was the most representative FFA released (3154%) during the gastric digestion of coconut oil samples. It was observed that the gastric digestion phase plays a crucial role in the MCFA lipolysis and the lipase activity restricted the amount of free MCFA liberated during the GI digestion, resulting in incomplete lipids hydrolysis. Ericsem Pereira thanks the São Paulo Research Foundation (FAPESP) for the scholarship and financial support (grants #2017/16979-7 and #2019/13008-6). The authors thank FAPESP (grants #2014/21252-0 and #2016/08566-1), and the National Council for Scientific and Technological Development (CNPq), (grants #305870/2014-9 and #406963/2016-9) for their financial support and scholarships. This study was also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior — Brazil (CAPES)—Finance Code 001 and supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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