Bioprocessing of sugarcane molasses into gluconic acid by Aureobasidium pullulans: effect of oxygen transfer rate in stirred and airlift bioreactors
| Autor: | Fernandes, Sílvia Mariana Vilaça, Dias, Bruna Filipa Silva, Belo, Isabel, Lopes, Marlene |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Popis: | The bioconversion of glucose to gluconic acid (GA) by Aureobasidium pullulans is an extremely oxygen-consuming bioprocess. The utilization of low-cost raw materials, instead of pure glucose or glucose syrup, is a step forward in the development of a sustainable process for GA production. For the first time, sugarcane molasses (ScM), which is approximately 20 times cheaper than pure glucose, was used as the substrate for GA production by A.pullulans in three bioreactors typology stirred tank (STR) operating at atmospheric pressure (400 rpm and 600 rpm), pressurized STR (1 bar and 4 bar of total air pressure), and airlift bioreactor (1 vvm and 2 vvm). For each bioreactor tested, the highest GA production was obtained in highly aerated cultures 600 rpm (STR), 4 bar (pressurized STR), and 2 vvm (airlift). Among the bioreactors, the highest GA titers were obtained in STR (67 g·L-1) and airlift (75 g·L-1), though the productivity was considerably lower in airlift. Simultaneously with GA synthesis, a considerable amount of fructooligosaccharides was produced in the first hours of the process, particularly in the airlift bioreactor (64 g·L-1). The findings of this work demonstrated, for the first time, the ability of A. pullulans to use ScM as a cheap substrate for the simultaneous production of GA and FOS, providing a novel strategy for the sustainable and economical production of both added-value compounds, according to the circular bioeconomy assumptions. Portuguese Foundation for Science and Technology (FCT) - UID/BIO/04469/2019, PhD grant SFRH/BD/129475/2017 and LABBELS-Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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