Enhanced glycerol conversion by Thermoanaerobacter strains
| Autor: | Magalhães, Carla Isabel Pereira, Ribeiro, Joaquim Alfredo França Martins, Guedes, Ana P., Arantes, Ana Luísa, Sousa, Diana Zita Machado, Stams, Alfons Johannes Maria, Alves, M. M., Cavaleiro, A. J. |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Popis: | Glycerol-rich waste streams produced as a surplus by the biodiesel industry can be treated and valorized by anaerobic microbial communities to produce biogas. Glycerol is a highly reduced compound. Its complete degradation to methane and carbon dioxide requires a syntrophic cooperation of anaerobic bacteria and archaea, either directly or through propionate, lactate or ethanol as intermediates. The aim of this work was to study glycerol valorization to methane by thermophilic microbial communities. Glycerol-degrading methanogenic communities were enriched at 55 ºC. A co-culture of Thermoanaerobacter and Methanothermobacter was obtained pointing to facultatively syntrophic glycerol degradation. This hypothesis was further tested by incubating Thermoanaerobacter brockii subsp. finnii and T. wiegelli type strains with glycerol (10 mmol L-1) in pure culture and with different hydrogenotrophic methanogens. The presence of the methanogen accelerated glycerol fermentation by the two Thermoanaerobacter strains up to 3.3 mmol L-1 day-1, corresponding to 12 times higher volumetric glycerol depletion rates in the methanogenic co-cultures than in pure bacterial cultures. The methanogen acted as a biological electron acceptor, which enhanced glycerol conversion by Thermoanaerobacter species, since it facilitates the redox balance and contributes to a higher energy gain of these bacteria. Therefore, syntrophic glycerol fermentation promotes faster anaerobic treatment of glycerol rich waste streams coupled to methane production. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|