| Autor: |
Mazumder, Mostafizur, Brechun, Katherine E., Kim, Yongjoo B., Hoffmann, Stefan A., Chen, Yih Yang, Keiski, Carrie-Lynn, Arndt, Katja Maren, McMillen, David R., Woolley, G. Andrew |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Popis: |
Light-switchable proteins offer numerous opportunities as tools for manipulating biological systems with exceptional degrees of spatiotemporal control. Most designed light-switchable proteins currently in use have not been optimised using the randomisation and selection/screening approaches that are widely used in other areas of protein engineering. Here we report an approach for screening light-switchable DNA-binding proteins that relies on light-dependent repression of the transcription of a fluorescent reporter. We demonstrate that the method can be used to recover a known light-switchable DNA-binding protein from a random library. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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