Photocontrol of Coiled-Coil Proteins in Living Cells
| Autor: | Zhang, Fuzhong Z., Timm, Katharina A., Arndt, Katja Maren, Woolley, G. Andrew |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Popis: | Light switching of the activity of a coiled-coil protein, the AP-1 transcription factor, in living cells was made possible by the introduction of a designed azobenzene-cross-linked dominant negative peptide, XAFosW (red and yellow in the picture). In the dark, XAFosW showed decreased helical content and decreased affinity for target Jun proteins (green); irradiation at 365 nm enhanced helicity and target affinity. |
| Databáze: | OpenAIRE |
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