| Autor: |
Rekecki, A., Gunasekara, R.A.Y.S.A., Dierckens, K., Laureau, S., Boon, N., Favoreel, H., Cornelissen, M., Sorgeloos, P., Ducatelle, R., Bossier, P., Van Den Broeck, W. |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Popis: |
The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V. anguillarum with sea bass larvae. In the present study, germ-free sea bass larvae were orally exposed to a V. anguillarum HI-610 pathogen labelled with the green fluorescent protein (GFP-HI-610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post-exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid- and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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