The Effect of Fenugreek on the Gene Expression of Arachidonic Acid Metabolising Enzymes

Autor: Varjas, Timea, Nowrasteh, Ghodratollah, Budán, Ferenc, Horváth, Gábor, Cseh, József, Gyöngyi, Zoltán, Makai, Sándor, Ember, István
Přispěvatelé: Institut of Public Health, University of Pécs Medical School (UP MS), University of Pecs-University of Pecs, Institute of Public Health, Department of Oncology, Szent György Country Hospital, Department of Medicinal and Aromatic Plants, University of West Hungary Agriculture Science Faculty
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Phytotherapy Research
Phytotherapy Research, Wiley, 2010, ⟨10.1002/ptr.3231⟩
ISSN: 0951-418X
1099-1573
DOI: 10.1002/ptr.3231⟩
Popis: International audience; The main bioactive compounds of Trigonella foenum graecum L. (fenugreek) seeds are protodioscin, trigoneoside, diosgenin, yamogenin, which have anti-carcinogenic potency through inhibition of cell proliferation and inhibition of prostaglandin synthesis. We examined the effect of fenugreek on ALOX and COX genes in AKR/J H-2k mice exposed to dimethylbenz[a]anthracene (DMBA), the potent carcinogen. Expression pattern of these genes was determined by detecting the mRNA expression in various tissues (the lungs, liver, spleen and the kidneys) in four groups of mice. Two groups were fed with normal and two of them with fenugreek containing nutriment. Each group divided into DMBA treated and control groups. Mice were autopsied on the 7th day after DMBA treatment for mRNA isolation. Fenugreek consumption itself did not change gene expression compared to the control group. DMBA could increase expression of ALOX12, ALOX15, ALOX5 genes mainly in all organs. Fenugreek consumption was generally protective in each organ in different manner. DMBA treatment increased COX2 gene expression, but fenugreek were protective in all tissues examined. In COX1 gene, fenugreek diet could suppress the expression, except for spleen, independently from carcinogen exposure. Therefore via inhibiting the arachidonic acid metabolism fenugreek may prevent tumorigenesis
Databáze: OpenAIRE
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