| Autor: |
Kotsias, M., Blanas, A., Vliet, S.J. van, Pirro, M., Spencer, D.I.R., Kozak, R.P. |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Popis: |
The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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