The production of alpha-cyclodextrin by enzymatic degradation of starch
| Autor: | Flaschel, E., Landert, J. P., Spiesser, D., Renken, A. |
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| Předmět: |
biosynthesis
Dextrins: BI metabolism Heat Hydrogen-Ion Concentration Kinetics Klebsiella pneumoniae: EN enzymology Protein Denaturation Research Su pharmacology Glucosyltransferases: ME Cyclodextrins: BI food and beverages 0 (Cyclodextrins) 0 (Dextrins) 0 (Fatty Alcohols) 0 (alpha-Cyclodextrins) EC 2.4.1.- (Glucosyltransferases) EC 2.4.1.19 (cyclomaltodextrin glucanotransferase) Fatty Alcohols: PD |
| Popis: | The maximum concentration of alpha-cyclodextrin for the enzymatic degradation of starch is limited to about 13.5 g X 1(-1). By addition of decanol, the equilibrium of the reaction system can be shifted towards an alpha-cyclodextrin yield of 50% even at high substrate concentrations. The main variables of the decanol process--pH, temperature, substrate quality, substrate, and enzyme concentration--have been studied. The cyclodextrin-glycosyltransferase from Klebsiella pneumoniae M5 al can preferentially be employed at pH 6 to 8, temperatures of 40 to 50 degrees C and a decanol concentration of 0.1 kg-1 starch. The dextrose equivalent of starch is important with respect to the maximum achievable starch concentration, but not with respect to the reaction. Under process conditions, the rate of alpha-cyclodextrin evolution is limited by the enzymatic reaction and not by mass transfer of decanol into the aqueous phase. [on SciFinder (R)] |
| Databáze: | OpenAIRE |
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