Effects of methotrexate on transfected DNA stability in mammalian cells
| Autor: | Pallavicini, M. G., DeTeresa, P. S., Rosette, C., Gray, J. W., Wurm, F. M. |
|---|---|
| Předmět: |
Tetrahydrofolate Dehydrogenase/genetics
animal structures viruses fungi Gene Expression Regulation/drug effects Methotrexate/*pharmacology Nucleic Acid Hybridization DNA Transfection Proto-Oncogene Proteins c-myc Mice Proto-Oncogene Proteins/genetics Recombinant/genetics Gene Amplification/*drug effects embryonic structures Proto-Oncogenes Animals DNA Probes |
| Popis: | The chromosomal locations, amounts, and level of expression of transfected, amplified c-myc and dihydrofolate reductase sequences were measured in cells cultured in the presence and absence of methotrexate. These studies show that the location and amount of transfected sequences, as well as the level of expression, were more variable when the cells were cultured in methotrexate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|