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The use of bacteriophages for controlling plant bacterial diseases is an increasing research field with great potential. Public concerns about environmental abuse of pesticides are increasing the popularity of organic production, that contributed to the growth of biological control agent studies, and their use in plant disease management. However, the efficient use of biocontrol agents requires good understanding of their biological characteristics and efficacy. Furthermore, phage therapy is an attractive option to prevent and control biofilm related infections, supposed to contribute to an improved success of such an integrated bacterial spot control strategy and to reduce the use of conventional pesticides, which is beneficial to the environment, and human and animal health. Phage therapy is carried out by countless positive aspects that facilitate the battle against bacterial infections. The study of these processes has provided important scientific background about new intermediates, unusual nanoparticles, different gene organizations and special regulatory mechanisms, thus expanding older disciplines such as biochemistry, genetics and microbiology. Several bacterial strains develop into resistant from different types of antibiotics; it is called multiple resistance antimicrobials if the resistance is for four or more different classes of antimicrobials. In this contest, my PhD project aimed to the engineer a lytic bacteriophage in order to overcome the antibiotic resistance problems. Moreover, based to the biofilm problem the idea is to use an anti-biofilm molecule in synergy with the bacteriophage. For this reason, my project was focused on the characterization of a bacteriophage, an anti-biofilm molecule, and a carrier that help the complexation. The first part of my PhD was dedicated on the isolation of Xanthomonas campestris pv. campestris-specific bacteriophage from soil. It was further characterized by studying its plaque morphology, host range, pH stability, and morphology. The second part of my project was focused on the detection of an antibiofilm molecule and its possible application in combination with the phage carried by the hydroxyapatite. The last part was on the study of the metabolic changed of the biofilm carried out by the phage and the anti-biofilm molecule. All these results helped for better understand the best way to engineer the phage for translate all the result carried out to other bacterial infection. |
