| Popis: |
This work is part of the wide field of research on cationic antimicrobial peptides (CAMPs), molecules of great therapeutical potential for their antibacterial action, directed also against multi-drug resistant strains. By using a panel of bioinformatic, experimental and computational techniques, three novel tools were developed: (1) a scoring function, which allows the identification of putative CAMPs inside protein sequences and permits to perform strain-specific researches; (2) a fusion construct for the expression of recombinant CAMPs, which allows to prepare high purity peptides of variable length in high yields; (3) methods for the modelling of CAMPs by means of the Monte Carlo strategy and implicit solvation energy functions. |
