Combination of a microfluidic device and fluorescence correlation spectroscopy to study peptide translocation across model membranes

Autor: Bachler, Simon, Lin, Chao-Chen, Dittrich, Petra S.
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
Popis: This paper reports a microfluidic device that allows for trapping, treatment, and analysis of up to 192 individual giant unilamellar vesicles (GUVs) to study the translocation of fluorescently labeled cell-penetrating peptides (CPPs) across model membranes by fluorescence correlation spectroscopy (FCS). The results indicate that the composition of the membrane influences the membrane permeability of the tested CPPs.
Databáze: OpenAIRE