| Autor: |
Heischmann, Svenja |
| Přispěvatelé: |
Leibfritz, Dieter, Christians, Uwe, Stohrer, Wolf-Dieter |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
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| Popis: |
Mycophenolic acid (MPA) is the cornerstone of most immunosuppressive drug regimens after organ transplantation. However, gastrointestinal side effects diminish MPA's otherwise outstanding safety and efficiency. Using a unique cell culture model featuring the human colon cancer cell line LS180 and a combination of molecular biology methods and a non-targeted proteo-metabolomic strategy based on high-performance liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy, we were able to identify impairment of LS180 cell lipid metabolism as well as effects on structural proteins and nucleotide-dependent processes as main mediators of the negative effects of MPA on gastrointestinal barrier function. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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