| Autor: |
Adamopoulos, Athanassios, Landskron, Lisa, Heidebrecht, Tatjana, Tsakou, Foteini, Bleijerveld, Onno B., Altelaar, Maarten, Nieuwenhuis, Joppe, Celie, Patrick H.N., Brummelkamp, Thijn R., Perrakis, Anastassis |
| Přispěvatelé: |
Crystal and Structural Chemistry, Afd Biomol.Mass Spect. and Proteomics, Sub Crystal and Structural Chemistry, Biomolecular Mass Spectrometry and Proteomics |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Popis: |
The cyclic enzymatic removal and ligation of the C-terminal tyrosine of α-tubulin generates heterogeneous microtubules and affects their functions. Here we describe the crystal and solution structure of the tubulin carboxypeptidase complex between vasohibin (VASH1) and small vasohibin-binding protein (SVBP), which folds in a long helix, which stabilizes the VASH1 catalytic domain. This structure, combined with molecular docking and mutagenesis experiments, reveals which residues are responsible for recognition and cleavage of the tubulin C-terminal tyrosine. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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