| Autor: |
Rizzo, M, Ducheyne, K D, Deelen, C, Beitsma, M, Cristarella, S, Quartuccio, M, Stout, T A E, de Ruijter-Villani, M |
| Přispěvatelé: |
LS Voortplanting Inwendige Ziekten, dES/dFAH FR, LS Klinische Reproductie |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Popis: |
BACKGROUND: Advanced mare age is associated with declining fertility and an increased risk of early pregnancy loss. Compromised oocyte quality is probably the primary reason for reduced fertility, but the defects predisposing to embryonic death are unknown. In women, advanced age predisposes to chromosome segregation errors during meiosis, which lead to embryonic aneuploidy and a heightened risk of miscarriage. OBJECTIVES: To evaluate the effect of advanced mare age on chromosome alignment and meiotic spindle morphology in in vitro matured (IVM) oocytes. STUDY DESIGN: Morphometric and morphological analysis. METHODS: To investigate differences in spindle organisation and chromosome alignment between young and old mares, oocytes collected from slaughtered mares were divided into two groups depending on mare age (young, ≤14 years; old, ≥16 years), IVM and stained to visualise chromatin and alpha-tubulin. Spindle morphology, morphometry and chromosome (mis)alignment were evaluated by confocal microscopy and 3D-image analysis. RESULTS: Oocytes from old mares showed a higher incidence of chromosome misalignment (47.4% vs. 4.5%; P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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