| Autor: |
Hoehl, Melanie Margarete, Weibert, Michael, Paust, Nils, Zengerle, Roland, Slocum, Alexander H., Steigert, Juergen |
| Přispěvatelé: |
Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Mechanical Engineering, Hoehl, Melanie Margarete, Slocum, Alexander H., Paust, Nils, Zengerle, Roland |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Zdroj: |
Melanie Hoehl |
| Popis: |
In this paper, we introduce a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA-extraction platform (LabTube). We demonstrate fully automated, fully closed extraction of as little as 100 DNA copies of verotoxin-producing (VTEC) Escherichia coli lysate in water, milk and apple juice in a standard laboratory centrifuge, followed by subsequent automatic LAMP amplification with an overall time-to-result of 1.5hrs. The system is disposable, fully closed and automated, requiring only a single pipetting step. The microcontroller-driven heating system is low-cost ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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