An in vitro model of mesenchymal stem cell targeting using magnetic particle labelling
| Jazyk: | angličtina |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Staining and Labeling Magnetic Phenomena Mesenchymal Stem Cells/cytology Cell Differentiation/genetics Biomarkers/metabolism Biological Endocytosis Dextrans/metabolism Gene Expression Regulation Models Osteogenesis/genetics Luminescent Measurements Humans Adipose Tissue/cytology Chondrogenesis/genetics Magnetite Nanoparticles Rheology |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 9(6):724-733 |
| ISSN: | 1932-7005 1932-6254 |
| Popis: | The specific targeting of cells to sites of tissue damage in vivo is a major challenge precluding the success of stem cell-based therapies. Magnetic particle-based targeting may provide a solution. Our aim was to provide a model system to study the trapping and potential targeting of human mesenchymal stem cells (MSCs) during in vitro fluid flow, which ultimately will inform cell targeting in vivo. In this system magnet arrays were used to trap superparamagnetic iron oxide particle-doped MSCs. The in vitro experiments demonstrated successful cell trapping, where the volume of cells trapped increased with magnetic particle concentration and decreased with increasing flow rate. Analysis of gene expression revealed significant increases in COL1A2 and SOX9. Using principles established in vitro, a proof-of-concept in vivo experiment demonstrated that magnetic particle-doped, luciferase-expressing MSCs were trapped by an implanted magnet in a subcutaneous wound model in nude mice. Our results demonstrate the effectiveness of using an in vitro model for testing superparamagnetic iron oxide particles to develop successful MSC targeting strategies during fluid flow, which ultimately can be translated to in vivo targeted delivery of cells via the circulation in a variety of tissue-repair models. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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