Feasibility of isotachochromatography as a method for the preparative separation of weak acids and weak bases. II. Experimental verification

Autor: Kooistra, C., Sluyterman, L.A.A.E.
Přispěvatelé: Chemical Engineering and Chemistry
Jazyk: angličtina
Rok vydání: 1988
Zdroj: Journal of Chromatography, 436(2), 181-189. Elsevier
ISSN: 0021-9673
Popis: The theory of isotachochromatography has been verified and proved to be essentially correct. Acetate was used as a leading buffer and mostly serine as a terminal buffer to separate acids with pKa values intermediate between those of the leading and terminal buffers on a column of Dowex 50W-X2. Separation could be effected. The length of a zone was proportional to the amount applied. The pH levels of the plateaux differed by values equal to the difference in the pKa values of the components as demanded by theory.
Databáze: OpenAIRE