| Autor: |
Marsman, Hendrik A., Heger, Michal, Kloek, Jaap J., Nienhuis, Syert L., van Werven, Jochem R., Nederveen, Aart J., ten Kate, Fiebo J. W., Stoker, Jaap, van Gulik, Thomas M. |
| Přispěvatelé: |
Other departments, Amsterdam Gastroenterology Endocrinology Metabolism, Cancer Center Amsterdam, Surgery, Gastroenterology and Hepatology, Amsterdam Cardiovascular Sciences, Amsterdam Neuroscience, Radiology and Nuclear Medicine, Pathology |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Zdroj: |
Journal of gastroenterology and hepatology, 26(2), 356-363. Wiley-Blackwell |
| ISSN: |
0815-9319 |
| Popis: |
Background and Aim: Living donors with marked (> 33%) macrovesicular steatosis (MaS) are excluded from living donor liver transplantation procedures. Experimental studies have shown that the development of steatosis can be prevented by supplementation with omega-3 fatty acids (FA), but no studies have investigated the reduction of steatosis using omega-3 FA. The aim of the present study was to investigate whether administration of omega-3 FA is effective in reducing steatosis. Methods: After fatty liver (FL) induction by a 3-week methionine/choline-deficient (MCD) diet, male Wistar rats were daily administered per gavage omega-3 FA (FL+Omega-3), omega-3-poor lipid solution (FL+Lipid), or NaCl (FL+NaCl) during 2 weeks. Control animals received standard chow without treatment. Determination of steatosis degree was performed before, during, and after treatment by clinical 3.0T (1) H-magnetic resonance spectroscopy ((1) H-MRS) and by histology and gas chromatography at the end of the 2-week treatment period. Results: Hepatic fat content ((1) H-MRS) was significantly reduced after 1 and 2 weeks of omega-3 FA treatment. Histological analysis revealed a mild (5-33%) MaS degree in omega-3-treated animals vs severe (> 66%) MaS in the FL+Lipid and FL+NaCl groups. Hepatic omega-6 : 3 FA ratio and total FA content were reduced in the FL+Omega-3 group. Furthermore, de novo lipogenesis (C16, C16 : 1ω7, C18 : 1ω9) was also lowered. The reduction in hepatic fat content was associated with decreased lobular inflammation and hepatic tumor necrosis factor- α and interleukin levels as well as an increased antioxidative capacity. Conclusion: Omega-3 FA are capable of reversing severe hepatic MaS and ameliorating pathophysiological features of non-alcoholic steatohepatitis such as hepatocellular damage, lobular inflammation, and a reduced antioxidative capacity |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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