Chromatofocusing. IV. Properties of an agarose poly(ethyleneimine) ion exchanger and its suitability for protein separations

Autor: Sluyterman, L.A.A.E., Wijdenes, J.
Přispěvatelé: Chemical Engineering and Chemistry
Jazyk: angličtina
Rok vydání: 1981
Zdroj: Journal of Chromatography, 206(3), 441-447. Elsevier
ISSN: 0021-9673
Popis: Polyethyleneimine has been attached to epoxide-activated Sepharose. The resulting material exhibits high capacity, fast equilibration on acid—base titration and no maxima in a pH gradient. The resolving power is improved when some of the imino groups are converted into guanidino groups on modification with O-methylisourea. The final material is suitable for separations at pH 9.2.
Databáze: OpenAIRE