The use of mass spectrometry in peptide chemistry
| Autor: | Leclercq, P.A., White, P.A., Hägele, K., Desiderio, D.M., Meienhofer, J. |
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| Přispěvatelé: | Chemical Engineering and Chemistry |
| Jazyk: | angličtina |
| Rok vydání: | 1972 |
| Zdroj: | Chemistry and biology of peptides : proceedings of the third American peptide symposium, 687-690 STARTPAGE=687;ENDPAGE=690;TITLE=Chemistry and biology of peptides : proceedings of the third American peptide symposium |
| Popis: | A review with 16 refs. Methods are detailed for derivatizing peptides (mg quantities) in order to provide sufficient volatility for mass spectrometry (at least 10-5 mm vapor pressure at 300 Deg is required). Three steps are used in producing the desired derivs.: (a) arginine side chains are converted to dimethylpyrimidyl ornithine by reaction with acetylacetone; (b) free amino groups are acetylated by reaction with Ac2O in order to avoid quaternization during permethylation; and (c) N, O, S permethylation is used to replace all H atoms not bound to C with a Me group by reacting the peptide with methylsulfinylmethide carbanion (DMSO-) to produce a peptide polyanion and then by adding MeI for methylation. With each mass spectrum obtained, the amino acid sequence is derived via a computer program similar to that of K. Biemann, et al. (1966). [on SciFinder (R)] |
| Databáze: | OpenAIRE |
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