| Autor: |
SINKE, RJ, SUIJKERBUIJK, RF, DEJONG, B, OOSTERHUIS, JW, VANKESSEL, AG |
| Jazyk: |
angličtina |
| Rok vydání: |
1993 |
| Předmět: |
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| Zdroj: |
GENES CHROMOSOMES & CANCER, 6(3), 161-165. Wiley |
| ISSN: |
1045-2257 |
| Popis: |
We present molecular data to demonstrate that the isochromosome 12p, specific for human germ cell tumors (GCTs), is of uniparental origin. Eight GCT-derived cell lines, containing one or more copies of i(12p) and/or other 12p anomalies, were analyzed with different 12p-derived polymorphic markers. The results from Ma-90, a near-diploid cell line with only one i(12p) in addition to two copies of a normal chromosome 12, clearly show an allelic 12p ratio of approximately 3:1, indicating that both 12p arms are of identical parental origin. These results were further substantiated by data obtained from the other i(12p)-positive GCT-derived cell lines. Therefore, we conclude that the i(12p) in GCTs constitutes a genuine isochromosome with genetically identical arms. The isochromosome most likely originates from a misdivision of the centromere rather than from a translocation or a non-sister chromatid exchange as proposed by others. We also found that supernumerary 12p copies, as observed in i(12p)-negative GCTs, are of uniparental origin. These observations seem to point to an important role for certain 12p-derived sequences in the development of human GCTs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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