| Autor: |
Roos, W.H., Vorselen, D., Van Dommelen, S.M., Van Loon, J.J.W.A., Mackintosh, F.C., Schiffelers, R.M., Wuite, G.J.L. |
| Přispěvatelé: |
Molecular Biophysics |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
European Biophysics Journal, 44(1). SPRINGER |
| ISSN: |
0175-7571 |
| Popis: |
Small and large unilamellar vesicles are ubiquitously present in cell biology, a prime example are extracellular vesicles (EVs). EVs are endogenous particles involved in cell to cell communication. EVs transport proteins and RNA, play a role in disease and are potentially useful as a drug delivery system. Their stiffness might be important for proper functioning because it is believed to play a role in e.g. endocytosis by cells. We look into the mechanics of (i) liposomes with complex lipid mixture and of (ii) EVs, using AFM imaging and nanoindentation [1]. We show that EVs (∼ 125 nm) stay in a spherical shape upon interaction with the surface, whereas similar sized liposomes adopt a hemi-spherical shape. Using the results of our nanoindentation approach, co-developed in my lab [2,3], we propose a new model to describe vesicle deformation. Based on Helfrich's theory, this model accurately describes the indentation behavior of both vesicle types. Furthermore, we show that osmotic pressure build-up during liposome spreading stiffens the response and that EVs have distinct mechanical properties from liposomes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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