| Autor: |
Morlot, C., Hemrika, W., Romijn, R.A., Gros, P., Cusack, S., McCarthy, A.A., Kristal- en structuurchemie, Dep Scheikunde |
| Rok vydání: |
2007 |
| Zdroj: |
Acta crystallographica. Section F, Structural biology and crystallization communications, F63, 689. Wiley-Blackwell |
| ISSN: |
1744-3091 |
| Popis: |
Activation of Roundabout 1 (Robo1) by Slit proteins results in axon repulsion from the midline. Robo1 is a large transmembrane receptor expressed on the axon growth cone and the minimal Robo1-binding region required for Slit activation has been mapped to the N-terminal Ig1-2 domains. The cDNA encoding the first two Ig domains of Robo1 has been cloned and the protein has been expressed in HEK293 EBNA-1 mammalian cells. Here, the purification and crystallization conditions of this Robo1 construct are reported. The crystals are orthorhombic, space group P21212, with unit-cell parameters a = 38.8, b = 69.4, c = 103.3 Å and one molecule in the asymmetric unit. X-ray diffraction data have been collected to 2.8 Å resolution on beamline ID29 at the ESRF. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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