Applications of CryoSEM in medical research
| Autor: | Stokroos, [No Value], Kalicharan, D, Jongebloed, WL, Robins, A |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Zdroj: | Nature, 30-30. Nature Publishing Group STARTPAGE=30;ENDPAGE=30;ISSN=0028-0836;TITLE=Nature |
| ISSN: | 0028-0836 |
| Popis: | The field emission scanning electron microscope (FE-SEM) allows imaging resolution clown to 0.5-1.5 nm, With this improved resolution, the cryo-preparation technique (Cryo-FE-SEWI) can now compete with freeze-fracture replication techniques for the transmission electron microscope (TEM) and with standard TEM techniques that use ultra-thin sections of biological tissue after chemical fixation. Here we compare cryo-prepared animal tissue with TEM images of the same biological structures that have been chemically fixed, dehydrated in ethanol, embedded in Epon and ultra-thin sectioned. |
| Databáze: | OpenAIRE |
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