Route of tracer administration does not affect real endogenous nitrogen recovery measured with the N-15-isotope dilution technique in pigs fed rapidly digestible diets

Autor: Steendam, CA, Verstegen, MWA, Tamminga, S, Boer, Huug, van 't End, M, Verstappen, B, Caine, WR, Visser, GH
Přispěvatelé: Isotope Research
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Zdroj: The Journal of Nutrition, 134(11), 3068-3075. Oxford University Press
ISSN: 0022-3166
Popis: The N-15-isotope dilution technique (N-15-IDT), with either pulse-dose oral administration or continuous i.v. administration of [N-15]-L-leucine (carotid artery), both at 5 mg/(kg body weight . d), was used to measure ileal (postvalve T-cecum cannula) endogenous nitrogen recovery (ENR) in pigs (9 +/- 0.6 kg). Diets were cornstarch, enzyme-hydrolyzed casein with no (control) or high (4%) content of quebracho extract (Schinopsis spp.) rich in condensed tannins. Blood was sampled from a catheter in the external jugular vein. Mean plasma N-15-enrichment at d 8-10 was higher (P = 0.0009) after i.v. than after oral administration [0.0356 vs. 0.0379 atom% excess (APE)]. Plasma 15N-enrichment for i.v. infused pigs was 0.01117 APE higher (P
Databáze: OpenAIRE