Rapid lamina propria retraction and zipper-like constriction of the epithelium preserves the epithelial lining in human small intestine exposed to ischaemia-reperfusion

Autor: Grootjans, Joep, Thuijls, Geertje, Derikx, Joep P. M., van Dam, Ronald M., Dejong, Cornelis H. C., Buurman, Wim A.
Přispěvatelé: Academic Medical Center
Jazyk: angličtina
Rok vydání: 2011
Zdroj: Journal of pathology, 224(3), 411-419. John Wiley and Sons Ltd
ISSN: 0022-3417
Popis: To ensure a sufficient barrier between a host and noxious luminal content, the intestinal epithelium must be equipped with efficient mechanisms to limit damage to the epithelial lining. Using a human model, we were able to investigate these mechanisms in the human gut exposed to ischaemia-reperfusion (IR) over the time course of 150 min. In 10 patients a part of jejunum, to be removed for surgical reasons, was selectively exposed to IR. Control tissue was collected, as well as tissue exposed to 30 min of ischaemia with 0, 30 or 120 min of reperfusion. Haematoxylin/eosin staining demonstrated the appearance of subepithelial spaces following 30 min of ischaemia, while the epithelial lining remained intact at this stage. Western blot for myosin light chain kinase (MLCK) revealed a significant increase in protein levels after ischaemia (p
Databáze: OpenAIRE
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