Het gebruik van kernspinresonantie (NMR) technieken bij de analyse van urine monsters

Autor:	Zomer G, Jacquemijns M, Stavenuiter JFC
Jazyk:	Dutch; Flemish
Rok vydání:	2012
Předmět:	nmr-spectrometry nmr-spectrometrie analysis analyse urine metabolites metabolieten
Popis:	1H NMR Spectroscopy yields within a relatively short time useful information on the presence of endogenous metabolites in rat urine. The very intense water signal can be attenuated by lyophilizing the samples and reconstitution of the sample in deuterated water, followed by a saturation pulse of the residual HOD signal. When lyophilized urine samples are compared with non-lyophilized samples no apparent artefacts are introduced. Homonuclear J-resolved 2D- NMR spectroscopy is very well suited to obtain additional structural information. Using this approach an unknown urinary metabolite was identified as N-methyl-3-carboxamide 4-pyridone.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=narcis______::99ebd6f95a8540c860845541a3d222c8 http://hdl.handle.net/10029/257952 Zobrazit plný text záznamu