| Autor: |
Timmermans, Sjoerd A. M. E. G., Damoiseaux, Jan G. M. C., Heerings-Rewinkel, Petra T. J., Ayalon, Rivka, Beck, Laurence H., Jr., Schlumberger, Wolfgang, Salant, David J., van Paassen, Pieter, Tervaert, Jan Willem Cohen |
| Přispěvatelé: |
Promovendi CD, MUMC+: DA CDL Algemeen (9), Med Microbiol, Infect Dis & Infect Prev, MUMC+: MA Nefrologie (9), MUMC+: MA Klinische Immunologie (9), Interne Geneeskunde, RS: CARIM - R3 - Vascular biology |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
American Journal of Clinical Pathology, 142(1), 29-34. American Society of Clinical Pathologists |
| ISSN: |
0002-9173 |
| Popis: |
Objectives: Autoantibodies against the M-type phospholipase A2 receptor 1 (anti-PLA2R1) have been demonstrated to be very specific for idiopathic membranous nephropathy (MN). We studied a novel enzyme-linked immunosorbent assay (ELISA) and compared results with results obtained using an indirect immunofluorescence (IF) and a Western blotting test (WB). Methods: One-hundred nine patients with idiopathic MN were recruited between November 1979 and March 2011. The control cohort comprised serum samples from patients with secondary MN (n = 16) and nephrotic controls (n = 17). The presence of anti-PLA2R1 in serum samples obtained at the time of renal biopsy was determined using ELISA, IIF, and WB. Results: With similar specificity (>= 97%), sensitivity varied from 68% (IIF) to 72% (ELISA, WB). Remarkably, patients who were seronegative for anti-PLA2R1 more often entered spontaneous remission (P = .038), whereas seropositive patients were more frequently treated with immunosuppressive agents (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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