| Autor: |
van Gurp, Thomas P, Wagemaker, Niels CAM, Wouters, Björn, Vergeer, Philippine, Ouborg, Joop NJ, Verhoeven, Koen JF |
| Přispěvatelé: |
Terrestrial Ecology (TE) |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Nature Methods, 13(4), 322-324. Nature Publishing Group |
| ISSN: |
1548-7091 |
| Popis: |
We describe epiGBS, a reduced representation bisulfite sequencing method for cost-effective exploration and comparative analysis of DNA methylation and genetic variation in hundreds of samples de novo. This method uses genotyping by sequencing of bisulfite-converted DNA followed by reliable de novo reference construction, mapping, variant calling, and distinction of single-nucleotide polymorphisms (SNPs) versus methylation variation (software is available at https://github.com/thomasvangurp/epiGBS). The output can be loaded directly into a genome browser for visualization and into RnBeads for analysis of differential methylation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink