| Autor: |
Koole, A, Bosman, J, Franke, JP, de Zeeuw, RA |
| Přispěvatelé: |
Groningen Research Institute of Pharmacy |
| Jazyk: |
angličtina |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography B, 726(1-2), 149-156 |
| ISSN: |
0378-4347 |
| Popis: |
Various beta(2)-agonists are used as illegal growth promoters in man and in animals. We developed a multiresidue procedure for the analysis of four beta-agonists in human and calf urine. The sample was pre-extracted with an Extrelut column at alkaline pH. The beta-agonists were eluted with a mixture of tert.-butylmethyl ether and hexane. Then the extract was further cleaned with a mixed mode SPE column, or with a combination of immunoaffinity chromatography (IAC) and the mixed mode SPE column. The IAC column contained antibodies against salbutamol, which were suitable for multiresidue extractions. The extract was then brought onto a mixed mode SPE column at an acidic pH. The column was washed with 70% methanol in water. Thereafter, the beta-agonists were eluted with ammoniated ethanol-hexane. The extract was analysed with an HPLC method with electrochemical detection. The beta-agonists were separated on a reversed-phase column using a mobile phase buffered at pH 5.5 and containing an ion-pair reagent. Recoveries were higher when the IAC procedure was not performed (90-105% vs. 65-75%), but the extracts were cleaner when the latter step was included. Detection limits in human and calf urine were in the low ng/ml range. The study indicated that beta(2)-agonists can be analysed in human and calf urine without the selectivity of a mass spectrometer, but that comprehensive clean-up is required to avoid the interference of urine matrix components. (C) 1999 Elsevier Science B.V. All rights reserved. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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