| Autor: |
CORVER, J, MOESBY, L, ERUKULLA, RK, REDDY, KC, BITTMAN, R, WILSCHUT, J |
| Přispěvatelé: |
Groningen University Institute for Drug Exploration (GUIDE) |
| Jazyk: |
angličtina |
| Rok vydání: |
1995 |
| Předmět: |
|
| Zdroj: |
Journal of Virology, 69(5), 3220-3223. American Society for Microbiology |
| ISSN: |
0022-538X |
| Popis: |
Low-pH-induced membrane fusion of Semliki Forest virus (SFV) in a model system is mediated by sphingolipids in the target membrane; ceramide is the sphingolipid minimally required (J. L. Nieva, R. Bron, J. Corver, and J. Wilschut, EMBO J. 13:2797-2804, 1994). Here, using various ceramide analogs, we demonstrate that sphingolipid-dependent fusion of SFV with cholesterol-containing liposomes exhibits remarkable molecular specificity, the 3-hydroxyl group and the 4,5-trans carbon-carbon double bond of the sphingosine backbone being critical for the sphingolipid to mediate the process. This observation supports the notion that sphingolipids act as a cofactor in SFV fusion, interacting directly with the viral fusion protein to induce its ultimate fusion-active conformation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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