Fusion of alphaviruses with liposomes is a non-leaky process

Autor: Smit, JM, Schoen, P, Corver, J, Bittman, R, Lin, KC, Wilschut, J
Přispěvatelé: Faculteit Medische Wetenschappen/UMCG
Jazyk: angličtina
Rok vydání: 2002
Předmět:
Zdroj: FEBS Letters, 521(1-3):PII S0014-5793(02)02823-5, 62-66. Wiley
ISSN: 9279-2928
0014-5793
Popis: It has been reported that low-pH-induced fusion of influenza virus with liposomes results in rapid and extensive release of both low- and high-molecular-weight substances from the liposomes [Gunther-Ausborn et al., J. Biol. Chem. 270 (1995) 29279-29285; Shangguan et al., Biochemistry 35 (1996) 4956-4965]. Here, we demonstrate retention of encapsulated water-soluble compounds during fusion of Semliki Forest virus (SFV) or Sindbis virus with liposomes at low pH. Under conditions allowing complete fusion of the liposomes, a limited fluorescence dequenching of liposome-encapsulated calcein was observed, particularly for SFV. Also, radioactively labeled inulin or sucrose were largely retained. Freezing and thawing of the viruses in the absence of sucrose resulted in an enhanced leakiness of fusion. These results support the notion that the alphavirus fusion event per se is non-leaky and may well involve a discrete hemifusion intermediate. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Databáze: OpenAIRE
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