Vascular endothelial growth factor overexpression in ischemic skeletal muscle enhances myoglobin expression in vivo
| Autor: | Weel, V. van, Deckers, M.M.L., Grimbergen, J.M., Leuven, K.J.M. van, Lardenoye, J.W.H.P., Schlingemann, R.O., Nieuw Amerongen, G.P. van, Bockel, J.H. van, Hinsbergh, V.W.M. van, Quax, P.H.A. |
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| Přispěvatelé: | Gaubius Instituut TNO |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Biomedical Research protein tyrosine kinase inhibitor beta galactosidase Gene Expression animal cell Mice Ischemia angiography pain Amputation Myoglobin messenger RNA adenovirus vector Gene Therapy RNA analysis Middle Aged semaxanib Female animal experiment Genetic Vectors Neovascularization Physiologic Muscle Fibers Adenoviridae in vivo study angina pectoris vascularization Animals Humans controlled study muscle ischemia gastrocnemius muscle RNA Messenger drug inhibition skeletal muscle Muscle Skeletal Biology protein expression mouse Aged nonhuman vasculotropin muscle function animal model capillary density Capillaries vasculotropin A Peripheral vascular disease myotube Angiogenesis Vascular endothelial growth factor |
| Popis: | Therapeutic angiogenesis using vascular endothelial growth factor (VEGF) is considered a promising new therapy for patients with arterial obstructive disease. Clinical improvements observed consist of improved muscle function and regression of rest pain or angina. However, direct evidence for improved vascularization, as evaluated by angiography, is weak. In this study, we report an angiogenesis-independent effect of VEGF on ischemic skeletal muscle, ie, upregulation of myoglobin after VEGF treatment. Mice received intramuscular injection with adenoviral VEGF-A or either adenoviral LacZ or PBS as control, followed by surgical induction of acute hindlimb ischemia at day 3. At day 6, capillary density was increased in calf muscle of Ad. VEGF-treated versus control mice (P |
| Databáze: | OpenAIRE |
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