| Autor: |
HAMOEN, LW, ESHUIS, H, JONGBLOED, J, VENEMA, G, VANSINDEREN, D |
| Přispěvatelé: |
Cardiovascular Centre (CVC) |
| Jazyk: |
angličtina |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Molecular Microbiology, 15(1), 55-63. Wiley |
| ISSN: |
0950-382X |
| Popis: |
The valine-activation domain-encoding portion of the srfA locus (srfA-d4) is not only involved in the non-ribosomal synthesis of surfactin, but is also required for the regulation of competence development. In this study we show that impairment of the adenylation activity of the valine-activating domain did not affect competence development. Deletion analysis and complementation studies delineated the competence-required portion of srfA-d4 to a 168 bp fragment, which contains a small open reading frame (ORF), designated comS, encoding a polypeptide of 46 amino acids, embedded within, but translated in, a frame different from that of srfA-d4. Introduction of an amber mutation in the comS-coding frame prevented competence development, demonstrating the involvement of comS in this prokaryotic specialization process. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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