Immersion oil for high-resolution live-cell imaging at 37°C: Optical and physical characteristics

Autor: Oomen, L.C.J.M., Sacher, R., Brocks, H.H.J., Zwier, J.M., Brakenhoff, G.J., Jalink, K.
Přispěvatelé: Molecular Cytology (SILS, FNWI)
Jazyk: angličtina
Rok vydání: 2008
Zdroj: Journal of Microscopy, 232(2), 353-361. Wiley-Blackwell
ISSN: 0022-2720
Popis: The use of normal immersion oil, developed for 23°C, at 37°C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37°C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23°C counterparts.
Databáze: OpenAIRE