The ECM deposited by basal asthmatic and non-asthmatic ASM cells is different in composition but not biological function

Autor: Harkness, L., Ashton, A., Burgess, J.
Přispěvatelé: Groningen Research Institute for Asthma and COPD (GRIAC), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Respirology, 20. Wiley
ISSN: 1323-7799
Popis: Aim: The remodelled asthmatic airway has increased airway smooth muscle cell (ASMC) growth, expanded vasculature, and altered extracellular matrix (ECM). The ECM is the external cellular microenvironment which regulates cell behaviour. Under proliferative, inflammatory, or fibrotic conditions, the asthmaticASM cells (AASMCs) deposit altered patterns of ECM proteins. However, it is unclear whether this is the case under basal, unstimulated conditions. This study aims to examine the composition of the ECM deposited by unstimulated NA (nonasthmatic)- and AASMCs, and biological potential for modulating blood vessel formation. Methods: Primary ASMCs from asthmatic and non-asthmatic individuals were quiesced in 0.1% BSA media after 72 hr of growth. After 24 hr ASMCs were lysed and the ECM either collected in lysate buffer and quantified using BCA, or kept intact and examined for collagen and fibronectin using Picrosirius Red and ELISA, respectively. Angiogenic potential was determined by examining Human Umbilical Vein Endothelial Cell (HUVEC) proliferation on (MTT and Cyquant assays), and attachment to ASMC-derived ECM. Unpaired t-tests were utilised to identify differences between NAASMC and AASMC-derived ECM. Results: Although the total amount of ECM deposited did not change (AASM N = 4 and NAASM N = 3 respectively), AASMCs deposited more collagen (N = 5, P
Databáze: OpenAIRE
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