Autor: |
Djavaheri, M., Mercado-Blanco, J., Versluis, C., Meyer, J.M., van Loon, L.C., Bakker, P.A.H.M., Biomolecular Mass Spectrometry and Proteomics, Plant Microbe Interactions, Dep Biologie, Sub Biomol.Mass Spectrometry & Proteom., Sub Plant-Microbe Interactions |
Jazyk: |
angličtina |
Rok vydání: |
2012 |
Předmět: |
|
Zdroj: |
MicrobiologyOpen, 1(3), 311. John Wiley and Sons Inc. |
ISSN: |
2045-8827 |
Popis: |
The plant growth-promoting rhizobacterium Pseudomonas fluorescens WCS374r produces several iron-regulated metabolites, including the fluorescent siderophore pseudobactin (Psb374), salicylic acid (SA), and pseudomonine (Psm), a siderophore that contains a SA moiety. After purification of Psb374 from culture supernatant of WCS374r, its structure was determined following isoelectrofocusing and tandem mass spectrometry, and found to be identical to the fluorescent siderophore produced by P. fluorescens ATCC 13525. To study the role of SA and Psm production in colonization of Arabidopsis thaliana roots and in induced systemic resistance (ISR) against Pseudomonas syringae pv. tomato (Pst) by strain WCS374r, mutants disrupted in the production of these metabolites were obtained by homologous recombination. These mutants were further subjected to transposon Tn5 mutagenesis to generate mutants also deficient in Psb374 production. The mutants behaved similar to the wild type in both their Arabidopsis rhizosphere-colonizing capacity and their ability to elicit ISR against Pst. We conclude that Psb374, SA, and Psm production by P. fluorescens WCS374r are not required for eliciting ISR in Arabidopsis. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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