| Autor: |
Valcek, Adam, Sismova, Petra, Nesporova, Kristina, Overballe-Petersen, Søren, Bitar, Ibrahim, Jamborova, Ivana, Kant, Arie, Hrabak, Jaroslav, Wagenaar, Jaap A, Madec, Jean-Yves, Damborg, Peter, van Duijkeren, Engeline, Ewers, Christa, Hordijk, Joost, Hasman, Henrik, Brouwer, Michael S M, Dolejska, Monika |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Popis: |
The relatedness of the equine-associated Escherichia coli ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of E. coli ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses across Europe [Czech Republic (n=23), the Netherlands (n=18), Germany (n=9), Denmark (n=3) and France (n=1)] from 2008-2017 were subjected to whole-genome sequencing. An additional 25 draft genome assemblies of E. coli ST1250 and ST1250-SLV/DLV were obtained from the public databases. The isolates were compared for genomic features, virulence genes, clade structure and plasmid content. The complete nucleotide sequences of eight IncHI1/ST9 and one IncHI1/ST2 plasmids were obtained using long-read sequencing by PacBio or MinION. In the collection of 79 isolates, only 10 were phylogenetically close (98% similarity) regardless of country of origin and varied only in the structure and integration site of MDR region. E. coli ST1250 and ST1250-SLV/DLV are phylogenetically-diverse strains associated with horses. A strong linkage of E. coli ST1250 with epidemic multi-drug resistance plasmid lineage IncHI1/ST9 carrying blaCTX-M-1 and the fos operon was identified. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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