Detection of Bartonella spp. DNA in clinical specimens using an internally controlled real-time PCR assay

Autor:	Bergmans, Anneke M C, Rossen, John W A
Přispěvatelé:	Microbes in Health and Disease (MHD)
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	DNA Bacterial hemic and lymphatic diseases Cats bacteria Animals Cat-Scratch Disease Humans Reference Standards bacterial infections and mycoses Bartonella Environment Controlled Real-Time Polymerase Chain Reaction Specimen Handling
Zdroj:	Methods in Molecular Biology, 943, 217-228
ISSN:	1064-3745
Popis:	Bartonella henselae is the causative agent of cat-scratch disease (CSD), usually presenting itself as a -self-limiting lymphadenopathy. In this chapter an internally controlled Taqman probe-based real-time PCR targeting the groEL gene of Bartonella spp. is described. This assay allows for the rapid, sensitive, and simple detection of Bartonella spp. in samples from CSD or endocarditis suspects, and it is suitable for implementation in the diagnostic microbiology laboratory.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=narcis______::18a747c16968f5cc87d3730e19690e9e https://research.rug.nl/en/publications/8db08925-c492-4858-82d0-45c9049a4700 Zobrazit plný text záznamu