Purification and characterization of a surface-binding protein from Lactobacillus fermentum RC-14 that inhibits adhesion of Enterococcus faecalis 1131

Autor: Heinemann, Christine, van Hylckama Vlieg, Johan E. T., Janssen, Dick B., Busscher, Henk J., van der Mei, Henny C., Reid, Gregor
Přispěvatelé: Biotechnology, Personalized Healthcare Technology (PHT), Man, Biomaterials and Microbes (MBM)
Jazyk: angličtina
Rok vydání: 2000
Předmět:
Zdroj: FEMS Microbiology Letters, 190(1), 177-180. Oxford University Press
ISSN: 0378-1097
Popis: Lactobacilli have been shown to be important in the maintenance of the healthy urogenital flora. One strain, Lactobacillus fermentum RC-14. releases surface-active components which can inhibit adhesion of uropathogenic bacteria. Using a quantitative method for determining inhibition of adhesion, a protein with high anti-adhesive properties against Enterococcus faecalis 1131 was purified. The N-terminal sequence of the 29-kDa protein was identical to that of a collagen-binding protein from Lactobacillus reuteri NCIB 11951, and exhibited close homology with a basic surface protein from L. fermentum BR11. The results suggest that this anti-adhesive cell surface protein
Databáze: OpenAIRE