| Autor: |
Berckmans, René J., Nieuwland, Rienk, Kraan, Maarten C., Schaap, Marianne C. L., Pots, Desirée, Smeets, Tom J. M., Sturk, Augueste, Tak, Paul P. |
| Přispěvatelé: |
Laboratory Specialized Diagnostics & Research, ACS - Amsterdam Cardiovascular Sciences, CCA -Cancer Center Amsterdam, Other Research, Clinical Immunology and Rheumatology, Laboratory for General Clinical Chemistry, AII - Amsterdam institute for Infection and Immunity |
| Jazyk: |
angličtina |
| Rok vydání: |
2005 |
| Zdroj: |
Arthritis research & therapy, 7(3), R536-R544. BioMed Central |
| ISSN: |
1478-6354 |
| Popis: |
Synovial fluid from patients with various arthritides contains procoagulant, cell-derived microparticles. Here we studied whether synovial microparticles modulate the release of chemokines and cytokines by fibroblast-like synoviocytes (FLS). Microparticles, isolated from the synovial fluid of rheumatoid arthritis (RA) and arthritis control (AC) patients (n = 8 and n = 3, respectively), were identified and quantified by flow cytometry. Simultaneously, arthroscopically guided synovial biopsies were taken from the same knee joint as the synovial fluid. FLS were isolated, cultured, and incubated for 24 hours in the absence or presence of autologous microparticles. Subsequently, cell-free culture supernatants were collected and concentrations of monocyte chemoattractant protein-1 (MCP1), IL-6, IL-8, granulocyte/macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF) and intracellular adhesion molecule-1 (ICAM-1) were determined. Results were consistent with previous observations: synovial fluid from all RA as well as AC patients contained microparticles of monocytic and granulocytic origin. Incubation with autologous microparticles increased the levels of MCP-1, IL-8 and RANTES in 6 of 11 cultures of FLS, and IL-6, ICAM-1 and VEGF in 10 cultures. Total numbers of microparticles were correlated with the IL-8 (r = 0.91, P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink