| Autor: |
Kerman, Niloufar Soltani, Qusai Hassan, Meissam Noroozifar, Kagan |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Chemosensors; Volume 11; Issue 6; Pages: 326 |
| ISSN: |
2227-9040 |
| DOI: |
10.3390/chemosensors11060326 |
| Popis: |
In this proof-of-concept study, gold nanoparticles (AuNPs) were immobilized on glassy carbon electrode (GCE) surfaces using a surface-anchored diazonium salt of 4-aminothiophenol (GCE-Ph-S-AuNPs). X-ray photoelectron spectroscopy (XPS) studies confirmed the attachment of the AuNPs via 4-thiophenol onto the surface of the modified electrode. Differential pulse voltammetry (DPV) was performed for the simultaneous determination of guanine (G) and 8-hydroxyguanine (8-OH-G). The calibration curves were linear up to 140 µM and 60 µM with a limit of detection of 0.02 µM and 0.021 µM for G and 8-OH-G, respectively. Moreover, chronoamperometric studies were carried out for the determination of diffusion coefficients of 8-OH-G and G. The GCE-Ph-S-AuNPs were also applied in genomic DNA-spiked samples for the determination of G and 8-OH-G with recovery rates between 98.5% and 103.3%. The novel electrochemical surface provided a potential platform for the sensitive detection of 8-OH-G related to oxidative stress-induced DNA damage in clinical studies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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