Identification and Functional Characterization of ZmSCYL2 Involved in Phytosterol Accumulation in Plants

Autor: Zhang, Chenchen Zhang, Wanlu Ma, Minyan Xu, Tao Li, Guomin Han, Longjiang Gu, Meng Chen, Mengting Zhang, Beijiu Cheng, Xin
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: International Journal of Molecular Sciences; Volume 24; Issue 12; Pages: 10411
ISSN: 1422-0067
DOI: 10.3390/ijms241210411
Popis: Phytosterols are natural active substances widely found in plants and play an important role in hypolipidemia, antioxidants, antitumor, immunomodulation, plant growth, and development. In this study, phytosterols were extracted and identified from the seed embryos of 244 maize inbred lines. Based on this, a genome-wide association study (GWAS) was used to predict the possible candidate genes responsible for phytosterol content; 9 SNPs and 32 candidate genes were detected, and ZmSCYL2 was identified to be associated with phytosterol accumulation. We initially confirmed its functions in transgenic Arabidopsis and found that mutation of ZmSCYL2 resulted in slow plant growth and a significant reduction in sterol content, while overexpression of ZmSCYL2 accelerated plant growth and significantly increased sterol content. These results were further confirmed in transgenic tobacco and suggest that ZmSCYL2 was closely related to plant growth; overexpression of ZmSCYL2 not only facilitated plant growth and development but also promoted the accumulation of phytosterols.
Databáze: OpenAIRE
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